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Subject: High-Dose Test: Overnight (8-mg) Test
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Dexamethasone (8 mg) is taken orally between 11 pm and midnight, and a single blood sample is drawn at 8 am the next day for measurement of serum cortisol.
With this protocol, the 8 am serum cortisol concentration is <5 μg/dL in most patients with Cushing disease (i.e., a pituitary tumor) and is usually undetectable in normals.