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Subject: Vitamin B2 (Riboflavin)
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Vitamin B2, or riboflavin, is one of the water-soluble vitamins. It is synthesized in plants and microorganisms and occurs naturally in three forms: the physiologically inactive riboflavin and the physiologically active coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). The latter accounts for about 90% of the total riboflavin in whole blood. Because of their capacity to transfer electrons, FAD and FMN are essential for proton transfer in the respiratory chain, for the dehydration of fatty acids, the oxidative deamination of amino acids, and for other redox processes.
Normal range: 3–15 μg/L.
Marginally low: 2 μg/L
Diminished: <2 μg/L
Evaluation of persons who present the signs of ariboflavinosis
Detect riboflavin deficiency
Patients with anorexia nervosa
Individuals who avoid dairy products (such as people with lactose intolerance) because dairy products are a good source of riboflavin
Patients with malabsorptive syndromes such as celiac sprue, malignancies, and short bowel syndrome
Rare inborn errors of metabolism in which there is a defect in riboflavin synthesis
Long-term use of phenobarbital and other barbiturates, which may lead to oxidation of riboflavin and impair its function
Testing of nonfasting specimens or the use of dietary vitamin B2 supplementation can result in elevated plasma vitamin B2 concentrations.
Sample should be frozen immediately to reduce the stability of B2 in serum.